国产精品ⅴ无码大片在线看,色综合久久一区二区三区,国产婷婷色一区二区三区在线,成人亚洲A片V一区二区三区蜜月

您好,歡迎進(jìn)入上海力敏實(shí)業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁 >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 抗體/蛋白 >> 4060SPhospho-Akt (Ser473) (D9E) XP®磷酸化Akt抗體

Phospho-Akt (Ser473) (D9E) XP®磷酸化Akt抗體

  • 更新時(shí)間:  2023-07-25
  • 產(chǎn)品型號(hào):  4060S
  • 簡(jiǎn)單描述
  • Phospho-Akt (Ser473) (D9E) XP®磷酸化Akt抗體
    WB IP IHC-P IHC-F IF-IC F
    H M R Hm Mk Dm Z B (C) (X) (Dg) (Pg
詳細(xì)介紹

Phospho-Akt (Ser473) (D9E) XP®磷酸化Akt抗體 #4060

PhosphoSitePlus mark.

PhosphoSitePlus® protein, site, and accession data: Akt1, Akt2, Akt3

No.SizePrice
4060L300 ul ( 60 western blots )please select country
4060S100 ul ( 20 western blots )please select country
4060P40 ul ( 8 western blots )please select country
4060carrier free & custom formulation / quantity request

 

ApplicationsReactivitySensitivityMW (kDa)Isotype
W IP IHC-P IHC-F IF-IC FH M R Hm Mk Dm Z B (C) (X) (Dg) (Pg)Endogenous60Rabbit IgG

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Dm=D. melanogaster X=Xenopus Z=Zebrafish B=Bovine Dg=Dog Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4060:

Flow, IHC / Frozen*, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting

* Product-specific protocol.

Specificity / Sensitivity

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb detects endogenous levels of Akt only when phosphorylated at Ser473.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser473 of human Akt.

Western Blotting

Western Blotting

Western blot analysis of extracts from PC-3 cells, untreated or LY294002/wortmannin-treated, and NIH/3T3 cells, serum-starved or PDGF-treated, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (left) or PTEN (138G6) Rabbit mAb #9559 (right). Note the presence of P-Akt staining in the PTEN deficient MDA-MB-468 cells.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma comparing SignalStain® Antibody Diluent #8112 (left) to TBST/5% normal goat serum (right) using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060.

 

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb on SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb.

 

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded PTEN heterozygous mutant mouse endometrium using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb. (Tissue section courtesy of Dr. Sabina Signoretti, Brigham and Women's Hospital, Harvard Medical School, Boston, MA.)

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded U-87MG xenograft, untreated (left) or lambda phosphatase-treated (right), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb.

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen SKOV3 xenograft using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb.

 

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, wortmannin and U0126 (blue), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells, LY294002-treated (left) or insulin-treated (right), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).

Background

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).

 

  1. Franke, T.F. et al. (1997) Cell 88, 435-7.
  2. Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
  3. Franke, T.F. et al. (1995) Cell 81, 727-36.
  4. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
  5. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
  6. Jacinto, E. et al. (2006) Cell 127, 125-37.
  7. Cardone, M.H. et al. (1998) Science 282, 1318-21.
  8. Brunet, A. et al. (1999) Cell 96, 857-68.
  9. Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4.
  10. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
  11. Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.
  12. Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.
  13. Cross, D.A. et al. (1995) Nature 378, 785-9.
  14. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
  15. Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.
  16. Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.
  17. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
  18. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
  19. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.

Application References

  • Gray, M.J. et al. (2008) J Natl Cancer Inst 100, 109-20. Applications: Western Blotting
  • Fos, C. et al. (2008) J Immunol 181, 1969-77. Applications: Western Blotting
  • Engelman, J.A. et al. (2008) Nat Med 14, 1351-6. Applications: IHC-P (paraffin)
  • Guertin, D.A. et al. (2009) Cancer Cell 15, 148-159. Applications: IHC-P (paraffin) Western Blotting
  • Allard, D. et al. (2008) J Biol Chem 283, 19739-47. Applications: IHC-P (paraffin)
  • De Raedt, T. et al. (2011) Cancer Cell 20, 400-13. Applications: Western Blotting
  • Kleiman, L.B. et al. (2011) Mol Cell 43, 723-37. Applications: Western Blotting
  • Sykes, S.M. et al. (2011) Cell 146, 697-708. Applications: Flow Cytometry
  • Cheung, R. et al. (2011) J Clin Invest 121, 4446-61. Applications: Western Blotting
  • Siemens, N. et al. (2011) J Biol Chem 286, 21612-22. Applications: Western Blotting
  • Lan, R. et al. (2012) Am J Physiol Renal Physiol , . Applications: IHC-P (paraffin)
  • Matsuura, S. et al. (2012) Blood , . Applications: Western Blotting
  • Jothi, M. et al. (2012) Cell Cycle 11, . Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

  • 4691 Akt (pan) (C67E7) Rabbit mAb
  • 2965 Phospho-Akt (Thr308) (C31E5E) Rabbit mAb
  • 8112 SignalStain® Antibody Diluent
  • 1140 Phospho-Akt (Ser473) Blocking Peptide
  • 2967 Akt1 (2H10) Mouse mAb
  • 4051 Phospho-Akt (Ser473) (587F11) Mouse mAb
  • 2964 Akt2 (5B5) Rabbit mAb
  • 3788 Akt3 (62A8) Rabbit mAb
  • 9272 Akt Antibody
  • 9916 Phospho-Akt Pathway Antibody Sampler Kit
  • 8101 SignalSlide® Phospho-Akt (Ser473) IHC Controls
  • 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
  • 7074 Anti-rabbit IgG, HRP-linked Antibody
  • 7720 Prestained Protein Marker, Broad Range (Premixed Format)
  • 7727 Biotinylated Protein Ladder Detection Pack
  • 7003 20X LumiGLO® Reagent and 20X Peroxide
  • 4071 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)
  • 4075 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
  • 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)

Phospho-Akt (Ser473) (D9E) XP®磷酸化Akt抗體產(chǎn)品詳細(xì)信息也可和選購

 

 


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
国产三级精品三级| 亚洲爆乳无码精品AAA片蜜桃| 夫妻那些事全集免费观看电视剧| 欧美人伦禁忌DVD放荡欲情| 亚洲国产精品无码久久98| 欧美三曰本三级少妇三99| 丰满少妇人妻久久久久久| 国内精品人妻无码久久久影院蜜桃 | 全免费a级毛片免费看视频| 国产精品国产三级区别第一集| 精品少妇一区二区三区视频 | 国产精品久久人妻互换| 人妻无码av中文系列久久| 国产精品成人精品久久久| 日韩AV在线观看| 国产探花在线精品一区二区| 国产香港明星裸体XXXX视频| 人妻丰满熟妞AV无码区| 国产精品成人va在线观看| a片在线观看免费| 国产69精品久久久久久人妻精品| 久久亚洲国产成人影院| 赵东苏菲花都兵王免费阅读 | 欧美性猛交XXXX乱大交| 中文字幕乱码人妻二区三区| 亚洲精品日韩一区二区电影| 精品无码一区二区三区亚洲桃色| 亚洲熟女乱综合一区二区| 欧美精品在线观看| a片毛片免费无限观看| 日韩欧美亚洲国产精品字幕久久久| 国产色视频一区二区三区| 免费精品国偷自产在线青年| 久久午夜伦鲁片免费无码| 精品人妻一区二区三区四区 | 国产玩弄人妻出轨系列| 男人激烈吮乳吃奶视频免费| 精品人妻一区二区三区四区| 免费看的www哔哩哔哩| 精品久久人人妻人人做精品 | 欧美人与性动交α欧美精品|