国产精品ⅴ无码大片在线看,色综合久久一区二区三区,国产婷婷色一区二区三区在线,成人亚洲A片V一区二区三区蜜月

您好,歡迎進(jìn)入上海力敏實(shí)業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁(yè) >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 常規(guī)生化試劑 >> B23111 B23112BIOTOOL代理 Poly FlAG多肽

BIOTOOL代理 Poly FlAG多肽

  • 更新時(shí)間:  2023-07-25
  • 產(chǎn)品型號(hào):  B23111 B23112
  • 簡(jiǎn)單描述
  • BIOTOOL代理 Poly FlAG多肽
    The Poly DYKDDDDK (FLAG) Peptide lyophilized powder was synthesized by 23 amino acid residues with molecular weight 2,864 Da. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three
詳細(xì)介紹

BIOTOOL代理 Poly FlAG多肽

Properties

Appearance (Color)White
Appearance (Form)Powder
FormLyophilized Powder
Purity by HPLC-MS95.33 % (the latest lot) Poly FLAG Peptide lyophilized powder
Concentration(Recommended working concentration is 100-400 μg/mL for elute FLAG fusion proteins from the Biotool Anti-DYKDDDDK (FLAG) affinity gel.
Shipped inBlue ice
Storage Temperature2-8°C

Application

For use in competitive elution of DYKDDDDK (FLAG) fusion proteins from the ANTI-FLAGmonoclonal antibody in solution or bound to agarose on the Biotool Anti-DYKDDDDK (FLAG) affinity gel.

Attention

1. Read the User Manual carefully before the first use;
2. Avoid freezing, drying and high-speed centrifugation during use and storage of beads;
3. This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Procedure

Peptide Elution of DYKDDDDK(FLAG) Fusion Protein from Biotool Anti-DYKDDDDK(FLAG) affinity gel

1. Thoroughly suspend the Anti-Flag Affinity Gel in the vial, for a uniform suspension of the resin. Quickly transfer 10μl of the gel suspension (about 5μl of packed gel volume) to a fresh tube.

2. Add 0.6 mL TBS. Thoroughly suspend the Anti-Flag Affinity Gel by pipetting. Centrifuge the resin at 5000 rpm for 30 secondsand carefully remove the supernatant. Be sure to remove all of the wash buffer without discarding the resin. Repeat 3-4 times.

3. Add 500 μL of cell periplasmic extracts to the washed resin.

4. Gently agitate samples for 2 hours at 4°C.
5. Centrifuge the resin for 30 seconds at 5000 rpm. Transfer the supernatants to a fresh tube.
6. Wash the resin with 0.5mL TBS until the OD280 of the supernatant reads<0.05.
7. Elution of DYKDDDDK (FLAG) Fusion Protein by Competition with Poly DYKDDDDK (FLAG) Peptide. Elute the bound DYKDDDDK(FLAG) Fusion Proteinby competitive elution with five one-column volume aliquots of a solution containing 100-400 ug/mL Poly DYKDDDDK(FLAG) Peptide in TBS. 
8. Recycling the Biotool Anti-DYKDDDDK(FLAG) affinity gel
Poly DYKDDDDK(FLAG) Peptidemay not elute all of the DYKDDDDK(FLAG) Fusion Protein bound to Biotool Anti-DYKDDDDK(FLAG) affinity gel. It is recommended the Biotool Anti-DYKDDDDK(FLAG) Affinity gel be regenerated immediay after use by washing with three 5 mL aliquots of 0.1 M glycine HCl, pH 3.5. The gel should be immediay re-equilibrated in TBS until the effluent is at neutral pH. 
Note: Do not leave the Biotool Anti-DYKDDDDK(FLAG) Affinity gel in glycine HCl for longer than 20 minutes.
9. Storing the Biotool Anti-DYKDDDDK(FLAG) Affinity gel
Wash the Biotool Anti-DYKDDDDK(FLAG) Affinity gel three times with 5 mL of 50% glycerol with 10mM sodium phosphate, 150 mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide. then add another 5 mL of 50% glycerol with 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azideand store at -20°C without draining.

Storage & stability

Store the product at 2-8°C.

File Download

Poly FLAG Peptide lyophilized powder

購(gòu)買此商品的顧客也同時(shí)購(gòu)買

HA多肽

HA多肽

c-Myc多肽

c-Myc多肽

V5多肽

V5多肽

Anti-DYKDDDDK(Flag)親和凝膠

Anti-DYKDDDDK(Flag)親和凝膠

Anti-HA親和凝膠

Anti-HA親和凝膠

Anti-Myc親和凝膠

Anti-Myc親和凝膠

Anti-V5親和凝膠

Anti-V5親和凝膠

抑制劑Cocktails

抑制劑Cocktails

蛋白純化和免疫沉淀的磁珠系列

蛋白純化和免疫沉淀的磁珠系列

Graphene Oxide

Graphene Oxide

 

 

BIOTOOL代理 Poly FlAG多肽

訂購(gòu)信息:

 

B23111 4mg

B23112 25mg


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
欧美黑人巨大xxxxx| 男女野外做爰电影免费| 丁香色欲久久久久久综合网| 亚洲精品乱码久久久久久| 97人妻一区二区精品免费| 亚洲H在线播放在线观看H| 国产成人无码AV片在线观看| 高潮久久久久久久久不卡| 免费看大片软件| 亚洲国产精品无码久久一区二区| 国产伦精品一区二区三区免费| 欧美乱熟人妻色情影视 | 麻豆婷婷狠狠色18禁久久| 亚洲精品熟女国产| 激情五月综合色婷婷一区二区| 99精品国产在热久久无码| 色欲AV自慰一区二区三区| 久久亚洲精品久久国产一区二区| 亚洲av色情成人永久网站小说| 精品无码人妻一区二区三区| 少妇伦子伦精品无码STYLES| 一区二区三区好用的精华液| 亚洲国产成人精品无码区在线网站 | 无码人妻av一二区二区三区| 欧美一区二区三区激情| 亚洲AV无码专区亚洲AV伊甸园| 国产精品丝袜黑色高跟鞋| 色偷偷国色天香在线观看免费视频| 国产重口老太和小伙A片| 欧美成人成人a片在线乱码视频| 国产CHINESE男男GAY片| 欧美XXXX性BBBBB喷水| 国产AV免费观看| 国产人久久人人人人爽| 成人禁片免费播放35分钟| 久久午夜无码鲁丝片秋霞| 久久黄色视频| 亚洲国产精品无码久久九九大片| 国产精品AV| 欧美做受又硬又粗又大视频| 国产欧美一区二区精品久久久|